국가연구개발성과
논문 (학술지)
Temporal regulation of ovine Interferon-tau Gene by the Transcription Factor EOMES in the Peri-Implantation Period
| 등록번호 | - | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
비SCI |
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| 저자명 (주·공동저자) | 김민수 ; 전익수; 김성우; 민관식; Kazuhiko Imakawa ※ 과제 참여정보와 일치하는 연구자 상세정보로 정확하지 않을 수 있습니다. 동일저자 논문보기 |
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| 논문구분 | 국외기타논문집 | 학술지명 | Biology of Reproduction |
| ISSN | 0006-3363 | 학술지 출판일자 | 2018-07-12 |
| 학술지 볼륨번호 | 51(0) | 논문페이지 | 242 ~ 242 |
| 학술지 임팩트팩터 | 3.432 | 기여율 | 100 % |
| 초록 | Expression Interferon tau (IFNT) regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, is essential for the maintenance of early pregnancy, but a definitive mechanism for its temporal transcription has not been elucidated. We and others have observed, the T-box protein eomesodermin (EOMES) exhibited high mRNA expression in the ovine embryonic trophectoderm; thus, CDX2, ETS2 and JUN coexist during the early stages of conceptus development. Objective of this study was to examine the effect of EOMES on ovine IFNT gene transcription when evaluated with CDX2, ETS2 and JUN transcription factors implicated in the control of cell differentiation in the trophectoderm. In this study, RT-qPCR analysis between ovine trophoblast cells was initially performed, finding that transcription factors Eomes and several transcription factor mRNAs were specific to trophectoderm cells. These mRNAs were also found in days 15, 17, and 21 ovine conceptuses. Furthermore, human choriocarcinoma JEG3 cells (trophoblast cell line) were co-transfected with an ovine IFNT (-654bp)-luciferase reporter (-654-oIFNT-Luc) construct and several transcription factor expression plasmids. Cotransfection of the reporter construct with CDX2, ETS2 and AP1(JUN) increased transcription of -654-oIFNT-Luc by about 11-fold compared with transfection of the construct alone. When cells were initially transfected with EOMES followed by transfection with CDX2, ETS2 and AP1(JUN), the expression of -654-oIFNT-Luc was decreased. Also, EOMES factor inhibited the stimulatory activity of CDX2 alone. These results suggest that when conceptuses attach to the uterine epithelium, ovine IFNT gene transcription is down-regulated by an increase of EOMES factor expression in the attached ovine trophoblast cells. | ||
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