국가연구개발성과
논문 (학술지)
Arenicin-1 elicits bacterial apoptotic like response Escherichia coli
| 등록번호 | - | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
비SCI |
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| 저자명 (주·공동저자) | 이희정 ; 이동건 ※ 과제 참여정보와 일치하는 연구자 상세정보로 정확하지 않을 수 있습니다. 동일저자 논문보기 |
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| 논문구분 | 국외기타논문집 | 학술지명 | - |
| ISSN | 0000-0000 | 학술지 출판일자 | - |
| 학술지 볼륨번호 | 0(0) | 논문페이지 | 1 ~ 10 |
| 학술지 임팩트팩터 | 0.0 | 기여율 | 100 % |
| 초록 | Arenicin-1, a 21-mer antimicrobial peptide, has been known to exert significant broad-spectrum antimicrobial activity with membrane active mechanisms. Bacterial mechanism need to elucidate further due to work several mechanisms for cell death. In the present study, the findings suggest a novel mechanism for the antibacterial effect of arenicin-1 on Escherichia coli, namely, a bacterial apoptosis-like death (ALD). We focused on a possible mechanism in arenicin-1-treated bacterial cells that includes the accumulation of reactive oxygen species (ROS), increased intracellular calcium levels, destruction of membrane potential, bacterial caspase-like protein activation, and DNA damage. Arenicin-1 induced ALD induced arenicin-1 includes the autocleavage of LexA due to RecA expression. A mutant strain of the RecA and LexA proteins, associated with the SOS response, was used to determine the necessity for RecA/LexA proteins in arenicin-1-mediated ALD. Furthermore, superoxide, hydrogen peroxide, and hydroxyl radicals, which are typical ROS, are removed from the cells treated with arenicin-1. Hydrogen peroxide interfere with the activity of arenicin-1 in E. coli, whereas superoxide and hydroxyl radical do not affect the activity of ALD induced by arenicin-1. Also, inhibition of Fenton reaction was attenuated the feature of ALD. Taken together, arenicin-1, which is required by RecA/LexA and is interrupted by hydrogen peroxide and Fenton reaction, induces ALD response in E. coli. The novel mechanisms of arenicin-1 may be an effective antimicrobial agent with far lower propensity for inducing microbial resistance than antibiotics. | ||
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