논문 (학술지)
Functional study of the cytochrome SlP450-72 gene isolated from tomato
| 등록번호 | RPMS-2020-0190807956 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
비SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Mai Thanh Dat; Park Seo Young; Ma Sang Hoon; Kim Ah Young; Kim Hyun Min; Joung Young Hee | ||
| 논문구분 | 국외기타논문집 | 학술지명 | In Vitro Cellular & Developmental Biology-Plant |
| ISSN | 1054-5476 | 학술지 출판일자 | 2018-08-19 |
| 학술지 볼륨번호 | 54 | 논문페이지 | S130 ~ S130 |
| 학술지 임팩트팩터 | 1.024 | 기여율 | 100 % |
| 초록 | Cytochrome P450 proteins are abundantly present in plants and required for the catalysis of many reactions, typically in production of primary and secondary metabolites. The functions of P450s normally involve the biochemical synthesis pathways comprise terpenoids, fatty acids, lipids, alkaloid, as well biosynthesis of plant hormones, especially, the catalysis of many products for chemical defense mechanism is their outstanding role. Tomato is well-known as one of plants contains numerous CYP genes most in their genome, but most of them have unknown function. To reveal their function, a P450 in CYP736A subfamily, CYP736A72 gene, was isolated from tomato (Solanum lycopersicum cv.Micro-Tom). To study the enzyme character of CYP736A72, this gene was heterologously expressed in E. coli, and the protein obtained after induction was used for analytical assays. The initial results show that this enzyme has 7-ethoxycoumarin O-deethylation activity. To identify CYP736A72’s function in plant, the transgenic lines of tomato and tobacco (Nicotiana tabacum cv.Xanthi-NC) which over-expressed CYP736A72 gene were produced and observed. | ||
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