논문 (학술지)
Transcriptome wide identification and characterization of NO-responsive WRKY transcription factors in Arabidopsis thaliana L.
| 등록번호 | RPMS-2019-0190778686 | SCI 구분
?
※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Imran Qari Muhammad; Hussain Adil; Mun Bong-Gyu; Lee Sang Uk; Asaf Sajjad; Ali Muhammad Amjad; Lee In-Jung; Yun Byung-Wook | ||
| 논문구분 | 국외전문학술지 | 학술지명 | ENVIRONMENTAL AND EXPERIMENTAL BOTANY |
| ISSN | 0098-8472 | 학술지 출판일자 | 2018-04-02 |
| 학술지 볼륨번호 | 148 | 논문페이지 | 128 ~ 143 |
| 학술지 임팩트팩터 | 4.369 | 기여율 | 100 % |
| DOI | 10.1016/j.envexpbot.2018.01.010 | ||
| 초록 | Abstract WRKY transcription factors are important plant-specific regulatory genes characterized by one or two conserved WRKY domain(s) usually followed by a zinc-finger motif. In this study using Arabidopsis thaliana , the RNA-Seq based transcriptomic analysis showed differential expression of 33 genes encoding WRKY TFs in response to the nitric oxide (NO) donor S-Nitrosocysteine (CySNO). Interestingly, 93.9% of these TFs were up-regulated with at least 2-fold change, suggesting their putative involvement in NO mediated gene regulation. GO- analysis of all the 33 transcriptomic elements showed their putative involvement in biological processes such as abiotic stress tolerance and defense against fungal pathogens (89.39 fold enrichment). Analysis of the NO-responsive AtWRKY TFs promoter region revealed the presence of the cis -acting elements such as ABRE, EIRE, ERE, and MBS involved in osmotic stress response, maximal elicitor-mediated activation, and drought-stress regulation. The analysis of NO-responsive AtWRKY TF motifs and their comparison with rice, soybean, and tomato orthologs suggested that members of the WRKY family belonging to the same group shared similar motifs and phylogenetic tree suggested that these TFs were highly conserved. Validation of transcriptomic data through quantitative real time-PCR showed a high correlation coefficient (0.85) indicating the high reliability and similarity of both types of analysis. Comparison of the NO-responsive and non-responsive WRKYs showed the presence of tyrosine (T) and cysteine (C) residues at a distance of 7 residues from the WRKYGQK motif which may serve as potential targets for modification by NO via tyrosine nitration and S-nitrosylation. We also validated the response of WRKYs through in vivo analysis using atwrky62 loss of function mutant and the results indicated a negative role of AtWRKY62 in plant growth. Furthermore, atwrky62 showed significantly less SNO contents compared to wild type plants indicating putative role of AtWRKY 62 in NO metabolism. Highlights The expression of more than 90% of the NO-responsive WRKY TFs was up-regulated within 6 h of CySNO infiltration. NO-responsive AtWRKY TFs share a high sequence homology with those of rice, tomato and soybean suggesting a similar conserved function for these WRKYs among the species. GO terms for biological processes associated majority of NO-responsive WRKYs to GO term for response to fungal stimuli. Most of the NO-responsive WRKY TFs have either Cysteine or Tyrosine residues (potential targets for protein S -nitrosylation) at 7 th position from WRKY motif while NO-non-responsive don’t have these residues. atwrky62 loss-of-function mutants negatively regulates shoot and root length under control and CySNO induced nitrosative stress. | ||
연구개발성과 등록 또는 활용에 대한 문의는 논문 연구개발성과 담당자를 통해 문의하시기 바랍니다.
[문의] 한국과학기술정보연구원 Tel : 042)716-7066, https://curation.kisti.re.kr/
- NTIS 관련 이용문의는 NTIS 콜센터(042-869-1115)로 문의하시기 바랍니다.
NTIS의 논문 정보는 국가연구개발사업 수행을 통해 발생된 성과로, 조사분석 등을 통해 입력된 정보를 수집 및 제공하고 있어, 출판사 또는 논문 정보 제공 사이트(Scienceon, RISS 등)에서 일괄 제공하는 논문 정보와 차이가 있을 수 있습니다.