논문 (학술지)
rvH1N1 Neuraminidase Inhibitory Activities of Phenolics from Perilla frutescens (L.) and Their Contents in Cultivars and Germplasm
| 등록번호 | RPMS-2019-0090809544 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
비SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Ha Tae Joung; Lee Myoung-Hee; Park Chang-Hwan; Kim Jung-In; Oh Eunyoung; Pae Suk-Bok; Park Jae Eun; Kim Sung-Up; Kwak Do-Yeon | ||
| 논문구분 | 국내전문학술지 | 학술지명 | Plant Breeding and Biotechnology |
| ISSN | 2287-9358 | 학술지 출판일자 | 2018-12-01 |
| 학술지 볼륨번호 | 6 | 논문페이지 | 404 ~ 412 |
| 학술지 임팩트팩터 | 0.6032 | 기여율 | 100 % |
| DOI | 10.9787/PBB.2018.6.4.404 | ||
| 초록 | The influenza neuraminidase (NA, E.C. 3.2.1.18), an antiviral, has been the target of high pharmaceutical companies due to its essential role in viral replication cycle. Perilla frutescens (P. frutescens) is used in traditional Chinese medicine for various diseases, such as cold due to wind-cold, headache and cough. In this context, four major polyphenolic compounds including rosmarinic acid-3-O-glucoside (1), rosmarinic acid (2), luteolin (3), and apigenin (4) isolated from P. frutescens were evaluated for their inhibitory effect on recombinant virus H1N1 neuraminidase (rvH1N1 NA). Among the test compounds, rosmarinic acid and luteolin inhibited the rvH1N1 NA with an IC50 of 46.7 and 8.4 μM, respectively. The inhibition kinetics analyzed by the Dixon plots indicated that rosmarinic acid and luteolin were noncompetitive inhibitors and that the inhibition constant, Ki, was established as 43.9 and 14.3 μM, respectively. In addition, 578 genetically diverse accessions and 39 cultivars of P. frutescens were analyzed using HPLC to characterize the diversity of polyphenolic composition and concentration. The individual and total compositions exhibited significant difference (p < 0.05), especially rosmarinic acid which was detected as the predominant metabolite in all accessions (58.8%) and cultivars (62.8%). Yeupsil and Sangback cultivars exhibited the highest rosmarinic acid (3,393.5 g/g) and luteolin (383.3 g/g) content respectively. YCPL177-2 with the high concentration (889.8 g/g) of luteolin may be used as a genetic resource for breeding elite cultivars. | ||
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