논문 (학술지)
One-Pot Multienzyme Cofactors Recycling (OPME-CR) System for Lactose and Non-natural Saccharide Conjugated Polyphenol Production
| 등록번호 | RPMS-2019-0190757430 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Darsandhari Sumangala; Pandey Ramesh Prasad; Shrestha Biplav; Parajuli Prakash; Liou Kwangkyoung; Sohng Jae Kyung | ||
| 논문구분 | 국외전문학술지 | 학술지명 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY |
| ISSN | 0021-8561 | 학술지 출판일자 | - |
| 학술지 볼륨번호 | 66 | 논문페이지 | 7965 ~ 7974 |
| 학술지 임팩트팩터 | 3.154 | 기여율 | 100 % |
| DOI | 10.1021/acs.jafc.8b02421 | ||
| 초록 | A one-pot multienzyme cofactors recycling (OPME-CR) system was designed for the synthesis of UDP-α-Dgalactose, which was combined with LgtB, a β-(1,4) galactosyltransferase from Neisseria meningitidis, to modify various polyphenol glycosides. This system recycles one mole of ADP and one mole of UDP to regenerate one mole of UDP-α-Dgalactose by consuming two moles of acetylphosphate and one mole of D-galactose in each cycle. The ATP additionally used to generate UDP from UMP was also recycled at the beginning of the reaction. The engineered cofactors recycling system with LgtB efficiently added a D-galactose unit to a variety of sugar units such as D-glucose, rutinose, and 2-deoxy-D-glucose. The temperature, pH, incubation time, and divalent metal ions for the OPME-CR system were optimized. The maximum number of UDP-α-D-galactose regeneration cycles (RCmax) was 18.24 by fed batch reaction. The engineered system generated natural and non-natural polyphenol saccharides efficiently and cost-effectively | ||
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