논문 (학술지)
Development and Characterization of Monoclonal Antibodies against Nucleoprotein for Diagnosis of Influenza A Virus
| 등록번호 | RPMS-2019-0090817613 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Nguyen Hong Phuong; | ||
| 논문구분 | 국내전문학술지 | 학술지명 | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY |
| ISSN | 1017-7825 | 학술지 출판일자 | 2018-05-28 |
| 학술지 볼륨번호 | 28 | 논문페이지 | 809 ~ 815 |
| 학술지 임팩트팩터 | 1.65 | 기여율 | 100 % |
| 초록 | Influenza, which is a highly contagious disease caused by the influenza A virus, continues to be a major health concern worldwide. Although the accurate and early diagnosis of influenza virus infection is important for controlling the spread of this disease and rapidly initiating antiviral therapy, the current influenza diagnostic kits are limited by their low sensitivity. In this study, we developed several new influenza nucleoprotein (NP)-specific monoclonal antibodies (mAbs) and compared their sensitivity and specificity of those with commercially available anti-NP mAbs. Three mAbs, designated M24.11, M34.3, and M34.33, exhibited higher reactivities to recombinant NPs and A/Puerto Rico/8/1934 (H1N1) viral lysates compared with the commercial mAbs, as assessed using enzyme-linked immunosorbent assays. M34.3 and M34.33 showed higher reactivities with A/California/04/09 (pandemic H1N1) and A/Philippines/2/82 (H3N2) viral lysates than the commercial mAbs. In contrast, M24.11 had marked reactivity with H3N2 but not with pandemic H1N1. Immunofluorescent confocal microscopy showed that the three mAbs effectively detected the presence of influenza virus in lung tissues of mice infected with A/Puerto Rico/8/1934. These results indicate that the newly developed M34.3 and M34.33 mAbs could be useful for the development of influenza diagnostics. | ||
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