논문 (학술지)
Structural and Functional Assessment of mBjAMP1, an Antimicrobial Peptide from Branchiostoma japonicum, Revealed a Novel alpha-Hairpinin-like Scaffold with Membrane Permeable and DNA Binding Activity
| 등록번호 | RPMS-2019-0190818354 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Nam Jiyoung; Yun Hyosuk; Rajasekaran Ganesan; Kumar S. Dinesh; Kim Jae Il; Min Hye Jung; Shin Song Yub; Lee Chul Won | ||
| 논문구분 | 국외전문학술지 | 학술지명 | JOURNAL OF MEDICINAL CHEMISTRY |
| ISSN | 0022-2623 | 학술지 출판일자 | 2018-12-27 |
| 학술지 볼륨번호 | 61 | 논문페이지 | 11101 ~ 11113 |
| 학술지 임팩트팩터 | 6.259 | 기여율 | 50 % |
| DOI | 10.1021/acs.jmedchem.8b01135 | ||
| 초록 | Here we describe the three-dimensional structure and antimicrobial mechanism of mBjAMP1, an antimicrobial peptide (AMP) isolated from Branchiostoma japonicum. The structure of mBjAMP1 was determined by 2D solution NMR spectroscopy and revealed a novel -hairpinin-like scaffold stabilized by an intramolecular disulfide bond. mBjAMP1 showed effective growth inhibition and bactericidal activities against pathogenic bacteria but was not cytotoxic to mammalian cells. Antimicrobial mechanism studies using fluorescence-based experiments demonstrated that mBjAMP1 did not disrupt membrane integrity. Laser-scanning confocal microscopy indicated that mBjAMP1 is able to penetrate the bacterial cell membrane without causing membrane disruption. Moreover, gel retardation assay suggested that mBjAMP1 directly binds to bacterial DNA as an intracellular target. Collectively, mBjAMP1 may inhibit biological functions by binding to DNA or RNA after penetrating the bacterial cell membrane, thereby causing cell death. These results suggest that mBjAMP1 may present a promising template for the development of peptide-based antibiotics. | ||
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