논문 (학술지)
Gene Expression Analysis and Polymorphism Discovery to Investigate Drought Responsive System in Tropical Maize
| 등록번호 | RPMS-2019-0090798205 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
비SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Song Kitae; Kim Hyo Chul; Kim Kyung-Hee; Moon Jun-Cheol; Kim Jae Yoon; Lee Sang-Kyu; Lee Byung-Moo | ||
| 논문구분 | 국내전문학술지 | 학술지명 | Plant Breeding and Biotechnology |
| ISSN | 2287-9358 | 학술지 출판일자 | 2018-12-01 |
| 학술지 볼륨번호 | 6 | 논문페이지 | 354 ~ 362 |
| 학술지 임팩트팩터 | 0.4359 | 기여율 | 100 % |
| DOI | 10.9787/PBB.2018.6.4.354 | ||
| 초록 | Maize has high food and industrial value, whereas has difficulties in research because of their 21 complex and huge size genome. Nested association mapping (NAM) was constructed to better 22 understand maize genetics. However, most studies were conducted using the reference genome 23 B73, and only a few studies were conducted on tropical maize. Ki3, one of the founder lines of 24 the NAM population, is a tropical maize. We analyzed the genetic characteristics of Ki3 by 25 using RNA sequencing and bioinformatics tools for various genetic studies. As results, a total 26 of 30,526 genes were expressed, and expression profile were constructed. A total of 1,558 27 genes were differentially expressed in response to drought stress, and 513 contigs of them come 28 from de novo assemblies. In addition, high-density polymorphisms including 464,930 single 29 nucleotide polymorphisms (SNPs), 21,872 multiple nucleotide polymorphisms (MNPs) and 30 93,313 insertions and deletions (InDels) were found compared to reference genome. Among 31 them, 15.0 % of polymorphisms (87,838) were passed non-synonymous test which could alter 32 amino acid sequences. The variants have 66,550 SNPs, 5,853 MNPs, and 14,801 InDels, also 33 proportion of homozygous type was higher than heterozygous. These variants were found in a 34 total of 15,643 genes. Of these genes, 637 genes were found as differentially expressed genes 35 (DEGs) under drought stress. Our results provide a genome-wide analysis of differentially 36 expressed genes and information of variants on expressed genes of tropical maize under 37 drought stress. Further characterization of these changes in genetic regulation and genetic traits 38 will be of great value for improvement of maize genetics. | ||
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