논문 (학술지)
Characterization of DNA comet and cellulose radical signal in Valencia oranges treated with different forms of ionizing radiation
| 등록번호 | RPMS-2019-0190777617 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Jo Y.; Sanyal B.; Ameer K.; Kwon J.H. | ||
| 논문구분 | 국외전문학술지 | 학술지명 | POSTHARVEST BIOLOGY AND TECHNOLOGY |
| ISSN | 0925-5214 | 학술지 출판일자 | 2018-01-01 |
| 학술지 볼륨번호 | 135 | 논문페이지 | 68 ~ 76 |
| 학술지 임팩트팩터 | 3.11 | 기여율 | 100 % |
| DOI | 10.1016/j.postharvbio.2017.08.004 | ||
| 초록 | Postharvest phytosanitary treatment of fruits with ionizing radiation has immense potential to promote international fruit trade. The effects of different forms of ionizing radiation on Valencia oranges stored for various durations were examined using a DNA comet assay, and radiation-induced radicals were quantified by electron spin resonance (ESR) spectroscopy. In non-irradiated seeds, the DNA appeared mostly undamaged, while irradiated samples had significantly longer DNA comet tail lengths. In contrast, DNA obtained from the flesh of the fruit was indistinguishable between the irradiated and non-irradiated samples. An extension in DNA comet tail length was observed as a function of storage time and applied irradiation dose. The ESR spectra of irradiated orangesdisplayedacharacteristictripletatg = 2.006withadistancebetweenextremepeaksof6mT.Irradiated samples could be distinguished from non-irradiated samples even after prolonged storage for 6 weeks. Finally, the results of these two assays were not affected by using different sources of ionizing radiation. Detection methods such as ESR and the DNA comet assay can be used to control the trade of oranges exposed to gamma rays, electron beam, and X-rays for quarantine applications. | ||
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