논문 (학술지)
Expression and Characterization of Calcium- and Zinc-Tolerant Xylose Isomerase from Anoxybacillus kamchatkensis G10
| 등록번호 | RPMS-2019-0090778766 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Park Yeong-Jun; Jerald | ||
| 논문구분 | 국내전문학술지 | 학술지명 | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY |
| ISSN | 1017-7825 | 학술지 출판일자 | 2018-04-01 |
| 학술지 볼륨번호 | 28 | 논문페이지 | 606 ~ 612 |
| 학술지 임팩트팩터 | 1.65 | 기여율 | 100 % |
| 초록 | The enzyme xylose isomerase (E.C. 5.3.1.5, XI) is responsible for the conversion of an aldose to ketose, especially xylose to xylulose. Owing to the ability of XI to isomerize glucose to fructose, this enzyme is used in the food industry to prepare high-fructose corn syrup. Therefore, we studied the characteristics of XI from Anoxybacillus kamchatkensis G10, a thermophilic bacterium. First, the gene coding for XI (xylA) was inserted into the pET-21a(+) expression vector and the construct was transformed into the Escherichia coli competent cell BL21 (DE3). The expression of recombinant XI was induced in the absence of isopropyl-thio-β- galactopyranoside and purified using Ni-NTA affinity chromatography. The optimum temperature of recombinant XI was 80oC and measurement of the heat stability indicated that 55% of residual activity was maintained after 2 h incubation at 60oC. The optimum pH was found to be 7.5 in sodium phosphate buffer. Magnesium, manganese, and cobalt ions were found to increase the enzyme activity; manganese was the most effective. Additionally, recombinant XI was resistant to the presence of Ca2+ and Zn2+ ions. The kinetic properties, Km and Vmax, were calculated as 81.44 mM and 2.237 μmol/min/mg, respectively. Through redundancy analysis, XI of A. kamchatkensis G10 was classified into a family containing type II XIs produced by the genera Geobacillus, Bacillus, and Thermotoga. These results suggested that the thermostable nature of XI of A. kamchatkensis G10 may be advantageous in industrial applications and food processing. | ||
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