논문 (학술지)
Tracking Down a New Steroid-Hydroxylating Promiscuous CytochromeP450: CYP154C8 from Streptomyces sp W2233-SM
| 등록번호 | RPMS-2018-0190698050 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Dangi Bikash; Ho; Oh Tae‐Jin | ||
| 논문구분 | 국외전문학술지 | 학술지명 | CHEMBIOCHEM |
| ISSN | 1439-4227 | 학술지 출판일자 | 2018-05-18 |
| 학술지 볼륨번호 | 19 | 논문페이지 | 1066 ~ 1077 |
| 학술지 임팩트팩터 | 2.77 | 기여율 | 0 % |
| DOI | 10.1002/cbic.201800018 | ||
| 초록 | CYP154C8 from Streptomyces sp. has been identified as a new cytochrome P450 with substrate flexibility towards different sets of steroids. In vitro treatment of these steroids with CYP154C8 revealed interesting product formation patterns with the same group of steroids. NMR study revealed the major product of corticosterone to be hydroxylated at the C21 position, whereas progesterone, androstenedione, testosterone, and 11-ketoprogesterone were exclusively hydroxylated at the 16α position. However, the 16α-hydroxylated product of progesterone was further hydroxylated to yield dihydroxylated products. 16-hydroxyprogesterone was hydroxylated at two positions to yield dihydroxylated products: 2α,16α-dihydroxyprogesterone and 6β,16α-dihydroxyprogesterone. To the best of our knowledge, this is the first report of generation of such products through enzymatic hydroxylation by a CYP450. In view of the importance of modified steroids as pharmaceutical components, CYP154C8 has immense potential for utilization in bioproduction of hydroxylated derivative compounds to be directly employed for pharmaceutical applications. | ||
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