논문 (학술지)
Improved production of 1-deoxynojirymicin in Escherichia coli through metabolic engineering
| 등록번호 | RPMS-2019-0190740453 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Rayamajhi Vijay; Dhakal Dipesh; Chaudhary Amit Kumar; Sohng Jae Kyung | ||
| 논문구분 | 국외전문학술지 | 학술지명 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY |
| ISSN | 0959-3993 | 학술지 출판일자 | 2018-05-23 |
| 학술지 볼륨번호 | 34 | 논문페이지 | 0 ~ 11 |
| 학술지 임팩트팩터 | 2.1 | 기여율 | 0 % |
| DOI | 10.1007/s11274-018-2462-3 | ||
| 초록 | Azasugars, such as 1-deoxynojirymicin (1-DNJ), are associated with diverse pharmaceutical applications, such as antidiabetic, anti-obesity, anti-HIV, and antitumor properties. Different azasugars have been isolated from diverse microbial and plant sources though complicated purification steps, or generated by costly chemical synthesis processes. But the biosynthesis of such potent molecules using Escherichia coli as a heterologous host provides a broader opportunity to access these molecules, particularly by utilizing synthetic biological, metabolic engineering, and process optimization approaches. This work used an integrated approach of synthetic biology, enzyme engineering, and pathway optimization for rational metabolic engineering, leading to the improved production of 1-DNJ. The production of 1-DNJ in recombinant E. coli culture broth was confirmed by enzymatic assays and mass spectrometric analysis. Specifically, the pathway engineering for its key precursor, fructose-6-phosphate, along with optimized media condition, results in the highest production levels. When combined, 1-DNJ production was extended to ~ 273 mg/L, which is the highest titer of production of 1-DNJ reported using E. coli | ||
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