논문 (학술지)
Ectopic serotonin production in beta-cell specific transgenic mice
| 등록번호 | RPMS-2018-0190674259 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Kim Hyeongseok; Kim Hyunki; Kim Kyuho; German Michael S.; Kim Hail | ||
| 논문구분 | 국외전문학술지 | 학술지명 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS |
| ISSN | 0006-291X | 학술지 출판일자 | 2018-01-08 |
| 학술지 볼륨번호 | 495 | 논문페이지 | 1986 ~ 1991 |
| 학술지 임팩트팩터 | 2.56 | 기여율 | 100 % |
| DOI | 10.1016/j.bbrc.2017.12.005 | ||
| 초록 | Genetically modified mice have been widely used in the field of β-cell research. However, analysis of results gathered using genetically modified organisms should be interpreted carefully as the results may be confounded by several factors. Here, we showed the ectopic serotonin (5-HT) production in β-cells of RIP-CreMgn, MIP-GFP, and MIP-Cre/ERT mice. These mice contained a human growth hormone (hGH) cassette to enhance transgene expression and hGH expression and Stat5 phosphorylation were detected in pancreatic islets of these mice. The expression level of tryptophan hydroxylase 1 (Tph1) was upregulated in pancreatic islets of transgenic mice with an hGH cassette but not in transgenic mice without an hGH cassette. Ectopic 5-HT production was not observed in β-cell-specific prolactin receptor (Prlr) knockout mice or Stat5 knockout mice crossed with RIP-CreMgn. We further confirmed that 5-HT production in β-cells of several transgenic mice was induced by hGH expression followed by the activation of the Prlr-Stat5-Tph1 pathway. These findings indicate that results obtained using transgenic mice containing the hGH cassette should be interpreted with care. | ||
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