논문 (학술지)
Optimization of Large-Scale Expansion and Cryopreservation of Human Natural Killer Cells for Anti-Tumor Therapy
| 등록번호 | RPMS-2019-0090716197 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Min Bokyung; Choi Hana; Her Jung Hyun; Jung Mi Young; Kim Hyo-Jin; Jung Mi-Young; Lee Eun-Kyoung; Cho Sung Yoo; Hwang Yu Kyeong; Shin Eui-Cheol | ||
| 논문구분 | 국외전문학술지 | 학술지명 | IMMUNE NETWORK |
| ISSN | 1598-2629 | 학술지 출판일자 | 2018-08-21 |
| 학술지 볼륨번호 | 18 | 논문페이지 | 0 ~ - |
| 학술지 임팩트팩터 | 2.52 | 기여율 | 0 % |
| DOI | 10.4110/in.2018.18.e31 | ||
| 초록 | Allogeneic natural killer (NK) cell therapy is a potential therapeutic approach for a variety of solid tumors. We established an expansion method for large-scale production of highly purified and functionally active NK cells, as well as a freezing medium for the expanded NK cells. In the present study, we assessed the effect of cryopreservation on the expanded NK cells in regards to viability, phenotype, and anti-tumor activity. NK cells were enormously expanded (about 15,000-fold expansion) with high viability and purity by stimulating CD3+ T cell-depleted peripheral blood mononuclear cells (PBMCs) with irradiated autologous PBMCs in the presence of IL-2 and OKT3 for 3 weeks. Cell viability was slightly reduced after freezing and thawing, but cytotoxicity and cytokine secretion were not significantly different. In a xenograft mouse model of hepatocellular carcinoma cells, cryopreserved NK cells had slightly lower anti-tumor efficacy than freshly expanded NK cells, but this was overcome by a 2-fold increased dose of cryopreserved NK cells. In vivo antibody-dependent cell cytotoxicity (ADCC) activity of cryopreserved NK cells was also demonstrated in a SCID mouse model injected with Raji cells with rituximab co-administration. Therefore, we demonstrated that expanded/frozen NK cells maintain viability, phenotype, and anti-tumor activity immediately after thawing, indicating that expanded/frozen NK cells can provide 'ready-to-use' cell therapy for cancer patients. | ||
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