논문 (학술지)
Quantitative Proteomic Analysis of 2D and 3D Cultured Colorectal Cancer Cells: Profiling of Tankyrase Inhibitor XAV939-Induced Proteome
| 등록번호 | RPMS-2019-0190739456 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Kim Young Eun; Jeon Hyo Jin; Kim Dahee; Lee Sun Young; Kim Ki Young; Hong Jongki; Maeng Pil Jae; Kim Kwang-Rok; Kang Dukjin | ||
| 논문구분 | 국외전문학술지 | 학술지명 | SCIENTIFIC REPORTS |
| ISSN | 2045-2322 | 학술지 출판일자 | - |
| 학술지 볼륨번호 | 8 | 논문페이지 | 13255 ~ - |
| 학술지 임팩트팩터 | 0.0 | 기여율 | 100 % |
| DOI | 10.1038/s41598-018-31564-6 | ||
| 초록 | Recently there has been a growing interest in three-dimensional (3D) cell culture systems for drug discovery and development. These 3D culture systems better represent the in vivo cellular environment compared to two-dimensional (2D) cell culture, thereby providing more physiologically reliable information on drug screening and testing. Here we present the quantitative profiling of a drug-induced proteome in 2D- and 3D-cultured colorectal cancer SW480 cells using 2D nanoflow liquid chromatography-tandem mass spectrometry (2D-nLC-MS/MS) integrated with isobaric tags for relative and absolute quantitation (iTRAQ). We identified a total of 4854 shared proteins between 2D- and 3D-cultured SW480 cells and 136/247 differentially expressed proteins (up/down-regulated in 3D compared to 2D). These up/down-regulated proteins were mainly involved in energy metabolism, cell growth, and cell-cell interactions. We also investigated the XAV939 (tankyrase inhibitor)-induced proteome to reveal factors involved in the 3D culture-selective growth inhibitory effect of XAV939 on SW480 cells. We identified novel XAV939-induced proteins, including gelsolin (a possible tumor suppressor) and lactate dehydrogenase A (a key enzyme of glycolysis), which were differentially expressed between 2D- and 3D-cultured SW480 cells. These results provide a promising informative protein dataset to determine the effect of XAV939 on the expression levels of proteins involved in SW480 cell growth. | ||
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