논문 (학술지)
Apoptotic and antiproliferative effects of Stigmast-5-en-3-ol from Dendronephthya gigantea on human leukemia HL-60 and human breast cancer MCF-7 cells
| 등록번호 | RPMS-2019-0190782723 | SCI 구분
?
※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Fernando I.P. Shanura; Sanjeewa K. K. Asanka; Ann Yong-Seok; Ko Chang-ik; Lee Seung-Hong; Lee Won Woo; Jeon You-Jin | ||
| 논문구분 | 국외전문학술지 | 학술지명 | TOXICOLOGY IN VITRO |
| ISSN | 0887-2333 | 학술지 출판일자 | 2018-10-01 |
| 학술지 볼륨번호 | 52 | 논문페이지 | 297 ~ 305 |
| 학술지 임팩트팩터 | 0.0 | 기여율 | 100 % |
| DOI | 10.1016/j.tiv.2018.07.007 | ||
| 초록 | Abstract The genus Dendronephthya encompasses marine soft corals that produce a wide spectrum of biofunctional terpenoids. Anticancer properties of these metabolites are widely exploited as potential chemotherapeutic agents. The present study reports the purification and isolation of a potential antiproliferative constituent, stigmast-5-en-3-ol from the 70% ethanol extract of the soft coral Dendronephthya gigantea . Among several other 3β-hydroxy-Δ5-steroidal congeners, stigmast-5-en-3-ol indicated prominent antiproliferative effects on HL-60 (leukemia) and MCF-7 (breast cancer) cell lines with IC 50 values of 37.82 and 45.17 μg/ml respectively. Stigmast-5-en-3-ol increased apoptotic body formation, accumulation of sub G1 apoptotic cells, and DNA damage in HL-60 and MCF-7 cells. It increased the expression of Bax, caspases, and PARP cleavage while decreasing Bcl-xL levels in both cancer cell lines indicating that the effects are arbitrated via the mitochondria-mediated apoptotic pathway. Steroidal derivatives were identified by GC MS/MS and the identity of stigmast-5-en-3-ol was confirmed by NMR spectra. The present study suggests that stigmast-5-en-3-ol could be a promising candidate for anticancer drug research. Highlights Stigmast-5-en-3-ol was isolated from a sterol rich fraction of Dendronephthya gigantea . Stigmast-5-en-3-ol decreased HL-60 and MCF-7 cancer cell proliferation. Stigmast-5-en-3-ol induced apoptosis via mitochondria-mediated pathway. Graphical abstract [DISPLAY OMISSION] | ||
연구개발성과 등록 또는 활용에 대한 문의는 논문 연구개발성과 담당자를 통해 문의하시기 바랍니다.
[문의] 한국과학기술정보연구원 Tel : 042)716-7066, https://curation.kisti.re.kr/
- NTIS 관련 이용문의는 NTIS 콜센터(042-869-1115)로 문의하시기 바랍니다.
NTIS의 논문 정보는 국가연구개발사업 수행을 통해 발생된 성과로, 조사분석 등을 통해 입력된 정보를 수집 및 제공하고 있어, 출판사 또는 논문 정보 제공 사이트(Scienceon, RISS 등)에서 일괄 제공하는 논문 정보와 차이가 있을 수 있습니다.