논문 (학술지)
Chronoamperometry-Based Redox Cycling for Application to Immunoassays
| 등록번호 | RPMS-2018-090683925 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Lee Ga-Yeon; Park Jun-Hee; Chang Young Wook; Cho Sungbo; Kang Min-Jung; Pyun Jae-Chul | ||
| 논문구분 | 국외전문학술지 | 학술지명 | ACS SENSORS |
| ISSN | 2379-3694 | 학술지 출판일자 | 2018-01-01 |
| 학술지 볼륨번호 | 3 | 논문페이지 | 106 ~ 112 |
| 학술지 임팩트팩터 | 0.0 | 기여율 | 17 % |
| DOI | 10.1021/acssensors.7b00681 | ||
| 초록 | In this work, the chronoamperometry-based redox cycling of 3,3′,5,5′-tetramethylbenzidine (TMB) was performed by using interdigitated electrode (IDE). The signal was obtained from two sequential chronoamperometric profiles: (1) with the generator at the oxidative potential of TMB and the collector at the reductive potential of TMB, and (2) with the generator at the reductive potential of TMB and the collector at the oxidative potential of TMB. The chronoamperometry-based redox cycling (dual mode) showed a sensitivity of 1.49 μA/OD, and the redox cycling efficiency was estimated to be 94% ( n = 10). The sensitivities of conventional redox cycling with the same interdigitated electrode and chronoamperometry using a single working electrode (single mode) were estimated to be 0.67 μA/OD and 0.18 μA/OD, respectively. These results showed that the chronoamperometry-based redox cycling (dual mode) could be more effectively used to quantify the oxidized TMB than other amperometric methods. The chronoamperometry-based redox cycling (dual mode) was applied to immunoassays using a commercial ELISA kit for medical diagnosis of the human hepatitis B virus surface antigen (hHBsAg). Finally, the chronoamperometry-based redox cycling (dual mode) provided more than a 10-fold higher sensitivity than conventional chronoamperometry using a single working electrode (single mode) when applied to a commercial ELISA kit for medical diagnosis of hHBsAg. Graphic Abstract | ||
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