논문 (학술지)
Novel imprinted single CpG sites found by global DNA methylation analysis in human parthenogenetic induced pluripotent stem cells
| 등록번호 | RPMS-2019-0190742320 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Choi Na Young; Bang Jin Seok; Lee Hye Jeong; Park Yo Seph; Lee Minseong; Jeong Dahee; Ko Kisung; Han Dong Wook; Chung Hyung-Min; Kim Gwang Jun; Shim Seung-Hyuk; Hwang Han Sung; Ko Kinarm | ||
| 논문구분 | 국외전문학술지 | 학술지명 | EPIGENETICS |
| ISSN | 1559-2294 | 학술지 출판일자 | 2018-05-03 |
| 학술지 볼륨번호 | 13 | 논문페이지 | 343 ~ 351 |
| 학술지 임팩트팩터 | 0.0 | 기여율 | 50 % |
| DOI | 10.1080/15592294.2018.1460033 | ||
| 초록 | Genomic imprinting is the process of epigenetic modification whereby genes are expressed in a parent-of-origin dependent manner; it plays an important role in normal growth and development. Parthenogenetic embryos contain only the maternal genome. Parthenogenetic embryonic stem cells could be useful for studying imprinted genes. In humans, mature cystic ovarian teratomas originate from parthenogenetic activation of oocytes; they are composed of highly differentiated mature tissues containing all three germ layers. To establish human parthenogenetic induced pluripotent stem cell lines (PgHiPSCs), we generated parthenogenetic fibroblasts from ovarian teratoma tissues. We compared global DNA methylation status of PgHiPSCs with that of biparental human induced pluripotent stem cells by using Illumina Infinium HumanMethylation450 BeadChip array. This analysis identified novel single imprinted CpG sites. We further tested DNA methylation patterns of two of these sites using bisulfite sequencing and described novel candidate imprinted CpG sites. These results confirm that PgHiPSCs are a powerful tool for identifying imprinted genes and investigating their roles in human development and diseases. | ||
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