논문 (학술지)
Nonintegrating Direct Conversion Using mRNA into Hepatocyte-Like Cells
| 등록번호 | RPMS-2019-0190724511 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Yoon Sangtae; Kang Kyojin; Cho Young-duck; Kim Yohan; Buisson Elina Maria; Yim Ji-Hye; Lee Seung Bum; Ryu Ki-Young; Jeong Jaemin; Choi Dongho | ||
| 논문구분 | 국외전문학술지 | 학술지명 | BIOMED RESEARCH INTERNATIONAL |
| ISSN | 2314-6133 | 학술지 출판일자 | 2018-09-20 |
| 학술지 볼륨번호 | 2018 | 논문페이지 | 0 ~ 0 |
| 학술지 임팩트팩터 | 0.0 | 기여율 | 33 % |
| DOI | 10.1155/2018/8240567 | ||
| 초록 | Recently, several researchers have reported that direct reprogramming techniques can be used to differentiate fibroblasts into hepatocyte-like cells without a pluripotent intermediate step. However, the use of viral vectors for conversion continues to pose important challenges in terms of genome integration. Herein, we propose a new method of direct conversion without genome integrationwith potential clinical applications. To generate hepatocyte-like cells,mRNA coding for the hepatic transcription factors Foxa3 and HNF4？？ was transfected into mouse embryonic fibroblasts. After 10-12 days, the fibroblasts converted to an epithelial morphology and generated colonies of hepatocyte-like cells (R-iHeps).ThegeneratedR-iHeps expressed hepatocyte-specificmarker genes and proteins, including albumin, alpha-fetoprotein, HNF4？, CK18, and CYP1A2. To evaluate hepatic function, indocyanine green uptake, periodic acid-Schiff staining, and albumin secretion were assessed. Furthermore, mCherry-positive R-iHeps were engrafted in the liver of Alb-TRECK/SCIDmice, andwe confirmed FAHenzyme expression in Fah1RTyrc/RJmodels. In conclusion, our data suggest that the nonintegratingmethod using mRNA has potential for cell therapy. | ||
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