논문 (학술지)
Clinical Assay for AFP-L3 by Using Multiple Reaction Monitoring-Mass Spectrometry for Diagnosing Hepatocellular Carcinoma
| 등록번호 | RPMS-2018-0190700073 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Kim Hyunsoo; Sohn Areum; Yeo Injoon; Yu Su Jong; Yoon Jung-Hwan; Kim Youngsoo | ||
| 논문구분 | 국외전문학술지 | 학술지명 | CLINICAL CHEMISTRY |
| ISSN | 0009-9147 | 학술지 출판일자 | 2018-01-01 |
| 학술지 볼륨번호 | 64 | 논문페이지 | 1230 ~ 1238 |
| 학술지 임팩트팩터 | 0.0 | 기여율 | 33 % |
| DOI | 10.1373/clinchem.2018.289702 | ||
| 초록 | BACKGROUND: Lens culinaris agglutinin-reactive fraction of α-fetoprotein (AFP-L3) is a serum biomarker for hepatocellular carcinoma (HCC). AFP-L3 is typically measured by liquid-phase binding assay (LiBA). However, LiBA does not always reflect AFP-L3 concentrations because of its low analytical sensitivity. Thus, we aimed to develop an analytically sensitive multiple reaction monitoring–mass spectrometry (MRM-MS) assay to quantify AFP-L3 in serum. METHODS: The assay entailed the addition of a stable isotope-labeled internal standard protein analog, the enrichment of AFP using a monoclonal antibody, the fractionation of AFP-L3 using L. culinaris agglutinin lectin, deglycosylation, trypsin digestion, online desalting, and MRM-MS analysis. The performance of the MRM-MS assay was compared with that of LiBA in 400 human serum samples (100 chronic hepatitis, 100 liver cirrhosis, and 200 HCC). Integrated multinational guidelines were followed to validate the assay for clinical implementation. RESULTS: The lower limit of quantification of the MRM-MS assay (0.051 ng/mL) for AFP-L3 was less than that of LiBA (0.300 ng/mL). Thus, AFP-L3, which was not observed by LiBA in HCC samples (n = 39), was detected by the MRM-MS assay, improving the clinical value of AFP-L3 as a biomarker by switching to a more analytical sensitive platform. The method was validated, meeting all the criteria in integrated multinational guidelines. CONCLUSIONS: Because of the lower incidence of false-negative findings, the MRM-MS assay is more suitable than LiBA for early detection of HCC. | ||
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