국가연구개발성과
논문 (학술지)
Single Oral Acute Toxicity of Banhasasim-Tang and Its Antiobesity Effect on Diet-Induced Obese Mice and 3T3-L1 Adipocytes
| 등록번호 | - | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
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| 저자명 (주·공동저자) | Yoo Sae-Rom; 유새롬; 하혜경 ; 정수진; 이미영; 신현규; 서창섭 ※ 과제 참여정보와 일치하는 연구자 상세정보로 정확하지 않을 수 있습니다. 동일저자 논문보기 |
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| 논문구분 | 국외전문학술지 | 학술지명 | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE |
| ISSN | 1741-427X | 학술지 출판일자 | - |
| 학술지 볼륨번호 | 0 | 논문페이지 | 0 ~ 0 |
| 학술지 임팩트팩터 | 2.064 | 기여율 | 100 % |
| DOI | 10.1155/2018/3865434 | ||
| 초록 | We had tested anti-obesity effect of 52 traditional herbal formulas in 3T3-L1 adipocyte, Banhasasim-tang (BHSST) was chosen as one of the effective medication to inhibit triglyceride accumulation. We investigated the anti-obesity effect of BHSST on 3T3-L1 adipocytes and high-fat diet (HFD)–induced obese mice. In addition, we evaluated the acute toxicity of BHSST in Sprague Dawley (SD) rats. Differentiated 3T3-L1 cells were treated with various concentrations of BHSST for 8 days. Accumulated triglyceride level and the expressions of adipogenesis-related genes and proteins were subsequently investigated. To evaluate the single oral toxicity of BHSST, the SD rats of each sex were administered a single dose (5000 mg/kg) of BHSST via oral gavage; the control group received vehicle only. After a single administration, the mortality, clinical signs, gross findings and body weight were monitored for 15 days. Male C57BL/6J mice were fed HFD for 4 weeks to induce obesity and randomly received 50 mg/kg of Orlistat (n=12, OR), 200 mg/kg of BHSST (n=12, B200), and 1000 mg/kg of BHSST (n=12, B1000) for another 8 weeks. BHSST suppressed the triglyceride contents and lipid accumulation in a dose-dependent manner in 3T3-L1 adipocytes. BHSST also downregulated the adipogenesis-related gene levels and protein expression compared with those in undifferentiated adipocytes. In a single oral dose toxicity study, there was no adverse effect on mortality, clinical signs, body weight changes and gross findings in the treatment group. HFD-fed mice treated with BHSST showed significantly reduced body weight gain, food efficiency ratio, and white adipose tissue weight. The medial lethal dose (LD50) of BHSST was 5000 mg/kg/day body weight for each sex in the rats. BHSST decreased the body weight gain in HFD-fed obese mice and inhibited triglyceride accumulation via a cascade of multiple factors at the mRNA and protein levels in 3T3-L1 adipocytes. | ||
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