논문 (학술지)
alpha-Solanine impairs oocyte maturation and quality by inducing autophagy and apoptosis and changing histone modifications in a pig model
| 등록번호 | RPMS-2018-0190687893 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Lin Tao; Oqani Reza K.; Lee Jae Eun; Kang Jeong Won; Kim So Yeon; Cho Eun Seok; Jeong Yong Dae; Baek Jun Jong; Jin Dong Il | ||
| 논문구분 | 국외전문학술지 | 학술지명 | REPRODUCTIVE TOXICOLOGY |
| ISSN | 0890-6238 | 학술지 출판일자 | 2018-01-31 |
| 학술지 볼륨번호 | 75 | 논문페이지 | 96 ~ 109 |
| 학술지 임팩트팩터 | 2.341 | 기여율 | 100 % |
| DOI | 10.1016/j.reprotox.2017.12.005 | ||
| 초록 | Abstract In this study, we used a pig model to investigate the effects of α-solanine (a natural toxin found mainly in potato sprouts) on oocyte maturation, quality and subsequent embryonic development. We found that α-solanine (10 muM) disturbed meiotic resumption and increased abnormal spindle formation and altered the cortical granule (CG) distribution compared with the untreated group. α-Solanine triggered autophagy and apoptosis by increasing the expressions of autophagy-related genes ( LC3 , ATG7 , and LAMP2 ) and apoptotic related genes ( BAX and CASP3 ). Exposure of porcine oocytes to α-solanine significantly increased the levels of H3K36me3 and H3K27me3. Moreover, α-solanine significantly reduced the cleavage and blastocyst formation rates, decreased the total and inner cell mass cells numbers, and increased apoptosis in these porcine embryos. Taken together, our data indicate that α-solanine toxically impairs oocyte maturation and quality by triggering autophagy/apoptosis and facilitating epigenetic modifications. Furthermore, α-solanine suppressed subsequent embryonic development and reduced embryo quality. Highlights During porcine IVM system, α-solanine disturbed meiotic resumption, induced morphological changes and death of oocytes. α-solanine increased the rates of abnormal spindle formation and cortical granule distribution. α-solanine triggered autophagy, apoptosis and ROS generation in pig oocytes. α-solanine changed the levels of H3K36me3 and H3K27me3 in pig oocytes. α-solanine exposure during IVM reduced the subsequent embryonic development and quality. | ||
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