논문 (학술지)
Schisandrin C enhances odontoblastic differentiation through autophagy and mitochondrial biogenesis in human dental pulp cells
| 등록번호 | RPMS-2018-0190690437 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Takanche Jyoti Shrestha; Kim Jeong-Seok; Kim Ji-Eun; Han S-H.; Yi Ho-Keun | ||
| 논문구분 | 국외전문학술지 | 학술지명 | ARCHIVES OF ORAL BIOLOGY |
| ISSN | 0003-9969 | 학술지 출판일자 | 2018-04-02 |
| 학술지 볼륨번호 | 88 | 논문페이지 | 60 ~ 66 |
| 학술지 임팩트팩터 | 1.748 | 기여율 | 100 % |
| DOI | 10.1016/j.archoralbio.2018.01.018 | ||
| 초록 | Objective: To investigate the role of Schisandrin C in odontoblastic differentiation, and its relations between autophagy and mitochondrial biogenesis in human dental pulp cells (HPDCs). Design: Fresh third molars were used, and cultured for HDPCs. Western blotting technique, Alizarin red S staining, alkaline phosphatase (ALP) activity, and confocal microscopy were used to detect autophagy, mitochondrial biogenesis, and odontoblastic differentiation. To understand the mechanism of Schisandrin C, the HDPCs were treated with lipopolysaccharide (LPS), autophagy and heme oxygenase-1 (HO-1) inhibitors: 3Methyladenine (3-MA) and Zinc protoporphyrin IX (ZnPP), respectively. Results: LPS decreased the expression of autophagy molecules [autophagy protein 5 (ATG-5), beclin-1, and microtubule-associated protein 1A/1B light chain 3 (LC3-I/II)] and mitochondrial biogenesis molecules [heme oxygenase-1 (HO-1) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α)], and disrupted odontoblastic differentiation. The down-regulation of autophagy and mitochondrial biogenesis with 3MA and ZnPP inhibited odontoblastic differentiation. However, Schisandrin C restored the expression of all the above molecules, even with LPS and inhibitor treatment. This result demonstrates that autophagy and mitochondrial biogenesis plays an essential role in odontoblastic differentiation, and Schisandrin C activates these systems to promote odontoblastic differentiation of HDPCs. Conclusion: Schisandrin C has potential characters to regulate odontoblastic differentiation, and may be recommended for use as a compound for pulp homeostasis. | ||
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