논문 (학술지)
Molecular cloning, purification, expression, and characterization of beta-1, 4-endoglucanase gene (Cel5A) from Eubacterium cellulosolvens sp isolated from Holstein steers' rumen
| 등록번호 | RPMS-2019-0190690815 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Park Tansol; Seo Seongwon; Shin Teaksoon; Cho Byung-Wook; Cho Seongkeun; Kim Byeongwoo; Lee Seyoung; Ha Jong K.; Seo Jakyeom | ||
| 논문구분 | 국외전문학술지 | 학술지명 | ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES |
| ISSN | 1011-2367 | 학술지 출판일자 | 2018-04-25 |
| 학술지 볼륨번호 | 31 | 논문페이지 | 607 ~ 615 |
| 학술지 임팩트팩터 | 0.971 | 기여율 | 100 % |
| DOI | 10.5713/ajas.17.0552 | ||
| 초록 | Objective: This study was conducted to isolate the cellulolytic microorganism from the rumen of Holstein steers and characterize endoglucanase gene (Cel5A) from the isolated microorganism. Methods: To isolate anaerobic microbes having endoglucanase, rumen fluid was obtained from Holstein steers fed roughage diet. The isolated anaerobic bacteria had 98% similarity with Eubacterium cellulosolvens (E. cellulosolvens) Ce2 (Accession number: AB163733). The Cel5A from isolated E. cellulolsovens sp. was cloned using the published genome sequence and expressed through the Escherichia coli BL21. Results: The maximum activity of recombinant Cel5A (rCel5A) was observed at 50°C and pH 4.0. The enzyme was constant at the temperature range of 20°C to 40°C but also, at the pH range of 3 to 9. The metal ions including Ca2+, K+, Ni2+, Mg2+, and Fe2+ increased the endoglucanase activity but the addition of Mn2+, Cu2+, and Zn2+ decreased. The Km and Vmax value of rCel5A were 14.05 mg/mL and 45.66 μmol/min/mg. Turnover number, Kcat and catalytic efficiency, Kcat/Km values of rCel5A was 96.69 (s–1) and 6.88 (mL/mg/s), respectively. Conclusion: Our results indicated that rCel5A of E. cellulosolvens isolated from Holstein steers had a broad pH range with high stability under various conditions, which might be one of the beneficial characteristics of this enzyme for possible industrial application. | ||
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