논문 (학술지)
Reusable and storable whole-cell microbial biosensors with a microchemostat platform for in situ on-demand heavy metal detection
등록번호 | RPMS-2018-0190699816 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
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저자명 (주·공동저자) | Bae Juyeol; 임지원 ※ 과제 참여정보와 일치하는 연구자 상세정보로 정확하지 않을 수 있습니다. |
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논문구분 | 국외전문학술지 | 학술지명 | SENSORS AND ACTUATORS B-CHEMICAL |
ISSN | 0925-4005 | 학술지 출판일자 | - |
학술지 볼륨번호 | 264 | 논문페이지 | 372 ~ 381 |
학술지 임팩트팩터 | 5.401 | 기여율 | 50 % |
초록 | Target analyte detection using whole-cell microbial biosensors can benefit from a microchemostat plat-form owing to the reduced reagent consumption, a finely controllable environment, and multi-parallelprocessing. However, the use of the previous microchemostat still has limitations for practical applica-tions, e.g., its single-use design, long time requirements for cell and device preparation, low portability,slow and weak response signals, and the lack of ready-to-use in situ devices. In the present study, wedeveloped a novel microchemostat platform that resolves these issues and enhances the performance ofmicrobial biosensors via its abilities to actively control the cell population and to form a uniform cultureenvironment using a nanoscale hydrodynamic film (NHF). The combination of the microchemostat plat-form and the microbial biosensors yielded fast and strong signals on demand in response to heavy metalions (e.g., Pb2+). In addition, the platform enables the long-term on-chip storage of microbial biosensorsfor over 1 month, without deterioration of growth and detection ability. Lastly, we demonstrated thatmicrobial biosensors can consecutively measure multiple samples by a regeneration process in which themicrochemostat platform continuously subcultures the microbial biosensors until the complete recoveryof their sensing capabilities. Thus, the microchemostat offers whole-cell microbial biosensors that arefast, ready-to-use, reusable, and storable. It also shows good potential for user-friendly, portable on-siteenvironmental monitoring, particularly where expensive analytical tools and/or instruments for heavymetal ion detection are unavailable. |
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