논문 (학술지)
Paraburkholderia azotifigens sp nov., a nitrogen-fixing bacterium isolated from paddy soil
| 등록번호 | RPMS-2020-0190700245 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Choi Gyu-Min; Im Wan-Taek | ||
| 논문구분 | 국외전문학술지 | 학술지명 | INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY |
| ISSN | 1466-5026 | 학술지 출판일자 | - |
| 학술지 볼륨번호 | 68 | 논문페이지 | 310 ~ 316 |
| 학술지 임팩트팩터 | 2.134 | 기여율 | 100 % |
| 초록 | A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strain, designated NF2-5-3 T , was isolated from a paddy soil in Anseong city, Republic of Korea. This bacterium was characterized to determine its taxonomic position using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NF2-5-3 T had a close relationship with, and was related most closely to, members of the genus Paraburkholderia, namely Paraburkholderia caribensis MWAP64T (98.8% similarity), P. sabiae Br3407 T (98.8 %), P. hospita LMG 20598 T (98.5 %), P. terrae NBRC 100964 T (98.3 %) and P. phymatum STM815 T (98.1 %). Growth of strain NF2-5-3 T occurred at 15-37 °C, at pH 6.0-8.0 and at NaCl concentrations of 0-2% (w/v). The major respiratory quinone was ubiquinone 8 (Q-8) and the major fatty acids were C 16 : 0 , summed feature 8 (comprising C 18 : 1 ω7c and/or C 18 : 1 ω6c), C 17 : 0 cyclo and C 16 : 0 3-OH. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, unidentified aminophospholipids, unidentified aminolipids and unidentified polar lipids. The G+C content of the genomic DNA was 64.2 mol%. DNA?DNA relatedness values between strain NF2-5-3 T and its closest phylogenetic neighbours were much lower than 70 %. Strain NF2-5-3 T could be differentiated phylogenetically and phenotypically from recognized species of the genus Paraburkholderia. The isolate therefore represents a novel species, for which the name Paraburkholderia azotifigens sp. nov. is proposed, with NF2-5-3 T (=KACC 18968 T = LMG 29961 T ) as the type strain. | ||
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