논문 (학술지)
Effect of Cytochalasin B Treatment on the Improvement of Survival Rate in Vitrified Pig Oocyte
| 등록번호 | RPMS-2019-0090823393 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
비SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Hwang In-Sul; Park Mi-Ryung; Kwak Tae-Uk; Park Sang-Hyun; Lim Ji-Hyun; Kim Sung Woo; Hwang Seongsoo | ||
| 논문구분 | 국내전문학술지 | 학술지명 | 발생과 생식 |
| ISSN | 2465-9525 | 학술지 출판일자 | 2018-09-30 |
| 학술지 볼륨번호 | 22 | 논문페이지 | 245 ~ 252 |
| 학술지 임팩트팩터 | 0.3971 | 기여율 | 100 % |
| DOI | 10.12717/DR.2018.22.3.245 | ||
| 초록 | To improve survival rates of vitrified pig oocytes, the treatment of cytoskeletal stabilizer on an appropriate time is one of the possible approaches. However, the exact treatment timing and effect of cytoskeletal stabilizer such as cyto-chalasin B (CB) is not well known during oocyte vitrification procedures. Thus, the present study was conducted to determine optimal treatment timing of CB during vitrification and warming procedures. In experiment 1, the survival rates of the post-warming pig oocytes were analyzed by fluorescein diacetate (FDA) assays with 4 classifications. In results, post-warming oo-cytes showed significantly (p<0.05) decreased number of alive oocytes (31.8% vs. 86.4%) compared to fresh control. In detail, the significant difference (p<0.05) was found only in strong fluorescence (18.2% vs. 70.5%) not in intermediate fluorescence groups (13.6% vs. 15.9%). In experiment 2, CB was treated before (CB-Vitri) and after (Vitri-CB) vitrification. In results, group of Vitri-CB showed significantly (p<0.05) higher (91.6%) survival rates compared to group of CB-Vitri (83.7%), sig-nificantly (p<0.05) and comparable with group of Vitri Control (88.7%) by morphological inspection. In FDA assay results, group of Vitri-CB showed significantly (p<0.05) higher (44.2%) survival rates compared to groups of CB-Vitri (36.7%) and Vitri Control (35.1%). In conclusion, the increased survival rates of post-warming pig oocyte treated with Vitri-CB method are firstly described here. The main finding of present study is that the CB treatment during recovery could be helpful to re-fresh the post-warming pig oocyte resulting its improved survival rates. | ||
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