논문 (학술지)
Effects of ice-binding protein from Leucosporidium on the cryopreservation of boar sperm
| 등록번호 | RPMS-2019-0090801782 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
비SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Park Sang Hyoun; Oh Keon Bong; Ock Sun-A; Byun Sung June; Lee Hwi-Cheul; Kumar Suresh; Lee Sung Gu; Woo Jae-Seok | ||
| 논문구분 | 국내전문학술지 | 학술지명 | 한국수정란이식학회지 |
| ISSN | 1225-4991 | 학술지 출판일자 | 2018-09-22 |
| 학술지 볼륨번호 | 33 | 논문페이지 | 185 ~ 194 |
| 학술지 임팩트팩터 | 0.0971 | 기여율 | 100 % |
| DOI | 10.12750/JET.2018.33.3.185 | ||
| 초록 | The aim of this study was performed to evaluate the effects of ice-binding protein from the arctic yeast Leucosporidium (LeIBP) supplementation on cryopreservation of boar sperm. The collected semen was diluted (1.5×108/ml) in lactose egg yolk (LEY) and cooled at 5°C for 3 h. The cooled semen was then diluted (1×108/ml) in LeIBP containing LEY with 9% glycerol and maintained at 5°C for 30 min. The semen was divided into six experimental groups (control, 0.001, 0.005, 0.01, 0.05 and 0.1 mg/ml of LeIBP). The straws were kept on above the liquid nitrogen (LN2) vapors for 20 minutes and then plunged into LN2. After thawing, computer-assisted sperm analysis was used for sperm motility and flow cytometry was performed to assess the viability, acrosome integrity (FITC-PSA/PI), ROS (DCFH-DA/PI), lipid peroxidation (BODIPY C11/PI) and apoptosis (Annexin V/PI), respectively. No significant responses were observed for sperm motility. However, sperm viability was significantly increased on 0.05 and 0.1 mg/ml of LeIBP groups compared to control (P < 0.05). In addition, acrosome integrity was significantly increases LeIBP groups (P < 0.05) and Both ROS and lipid peroxidation level were lower in all LeIBP groups than that of control (P < 0.05). On the other hand, a significant higher apoptosis rate was observed in 0.05 and 0.1 mg/ml of LeIBP groups compared to control (P < 0.05). It can be assumed that a supplementation of LeIBP in boar sperm freezing extender is an effective method to increase the sperm qualities after cryopreservation. | ||
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