논문 (학술지)
Solid cultures of thrips-pathogenic fungi Isaria javanica strains for enhanced conidial productivity and thermotolerance
| 등록번호 | RPMS-2019-0290802686 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Park So Eun; Kim Jong Cheol; Lee Se Jin; Lee Mi Rong; Kim Sihyeon; Li Dongwei; Baek Sehyeon; Han Ji Hee; Kim Jeong Jun; Koo Kyung Bon; Shin Tae Young; Kim Jae Su | ||
| 논문구분 | 국외전문학술지 | 학술지명 | Journal of Asia-Pacific Entomology |
| ISSN | 1226-8615 | 학술지 출판일자 | - |
| 학술지 볼륨번호 | 21 | 논문페이지 | 1102 ~ 1109 |
| 학술지 임팩트팩터 | 1.046 | 기여율 | 100 % |
| DOI | 10.1016/j.aspen.2018.08.005 | ||
| 초록 | Entomopathogenic fungi have great potential to control agricultural and horticultural insect pests, however optimizing conidial production systems to demonstrate high productivity and stability still needs additional efforts for successful field application and industrialization. Although many virulent entomopathogenic fungal isolates have been viewed as potential candidates in a laboratory environment, very few of the isolates are being used in practice for application in agricultural fields as commercial products. I. javanicus is an entomopathogenic fungus that is parasitic to various diverse coleopteran and lepidopteran insects and thought good candidate as biopesticdes. In this work, the basic characteristics of two entomopathogenic fungi, I. javanica FG340 and Pf04, were investigated in morphological examinations, genetic identification, and virulence against Thrips palmi, and then the feasibility of various grains substrates for conidial production was assessed, particularly focusing on conidial productivity and thermotolerance. Isaria javanica FG340 and Pf04 conidia were solid-cultured on 12 grains for 14 days in a Petri dish. Of the tested Italian millet, perilla seed, millet and barley-based cultures showed high conidial production. The four-grain media yielded more than 1 × 109conidia/gofI. javanica FG340 and Pf04. Pf04 strain had enhanced thermotolerance up to 45℃ when cultured on Italian millet. In application, it was easy to make a conidial suspension using the cultured grains, and several surfactants were tested to release the conidia. This work suggests several possible inexpensive grain substrates by which to promote conidial production combined with enhanced stability against exposure to high temperature. | ||
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