논문 (학술지)
Structured illumination microscopy imaging reveals localization of replication protein A between chromosome lateral elements during mammalian meiosis
| 등록번호 | RPMS-2019-0090727305 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Yoon Seobin; Choi Eui-Hwan; Kim Jung-Woong; Kim Keun P. | ||
| 논문구분 | 국외전문학술지 | 학술지명 | EXPERIMENTAL AND MOLECULAR MEDICINE |
| ISSN | 1226-3613 | 학술지 출판일자 | 2018-08-28 |
| 학술지 볼륨번호 | 50 | 논문페이지 | 0 ~ 12 |
| 학술지 임팩트팩터 | 5.063 | 기여율 | 33 % |
| DOI | 10.1038/s12276-018-0139-5 | ||
| 초록 | An important event enabling meiotic prophase I to proceed is the close juxtaposition of conjoined chromosome axes of homologs and their assembly via an array of transverse filaments and meiosis-specific axial elements into the synaptonemal complex (SC). During meiosis, recombination requires the establishment of a platform for recombinational interactions between the chromosome axes and their subsequent stabilization. This is essential for ensuring crossover recombination and proper segregation of homologous chromosomes. Thus, well-established SCs are essential for supporting these processes. The regulation of recombination intermediates on the chromosome axis/SC and dynamic positioning of double-strand breaks are not well understood. Here, using super-resolution microscopy (structured illumination microscopy), we determined the localization of the replication protein A (RPA) complex on the chromosome axes in the early phase of leptonema/zygonema and within the CEs of SC in the pachynema during meiotic prophase in mouse spermatocytes. RPA, which marks the intermediate steps of pairing and recombination, appears in large numbers and is positioned on the chromosome axes at the zygonema. In the pachynema, RPA foci are reduced but do not completely disappear; instead, they are placed between lateral elements. Our results reveal the precise structure of SC and localization dynamics of recombination intermediates on meiocyte chromosomes undergoing homolog pairing and meiotic recombination. | ||
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