논문 (학술지)
Silicon transporter genes of Fragilariopsis cylindrus (Bacillariophyceae) are differentially expressed during the progression of cell cycle synchronized by Si or light
| 등록번호 | RPMS-2019-0090784863 | SCI 구분
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※구분 : SCI(SCIE포함), 비SCI |
SCI |
|---|---|---|---|
| 저자명 (주·공동저자) | Oh Han Sang; Lee Sung-eun; Han Chae-seong; Kim Joon; Nam Onyou; Seo Seungbeom; Chang Kwang Suk; Jin EonSeon; Hwang Yong-sic | ||
| 논문구분 | 국외전문학술지 | 학술지명 | ALGAE |
| ISSN | 1226-2617 | 학술지 출판일자 | - |
| 학술지 볼륨번호 | 33 | 논문페이지 | 191 ~ 203 |
| 학술지 임팩트팩터 | 1.367 | 기여율 | 50 % |
| DOI | 10.4490/algae.2018.33.5.8 | ||
| 초록 | Fragilariopsis cylindrus is one of the most successful psychrophiles in the Southern Ocean. To investigate the molecular mechanism of biomineralization in this species, we attempted to synchronize F. cylindrus growth, since new cell wall formation is tightly coupled to the cell division process. Nutrient limitation analysis showed that F. cylindrus cultures rapidly stopped growing when deprived of silicate or light, while growth continued to a certain extent in the absence of nitrate. Flow cytometry analysis indicated that deprivation of either silicate or light could effectively arrest the cell cycle of this diatom species at the G1 phase, suggesting that synchrony can be established using either factor. Fluorescence labeling of new cell walls was faintly detectable as early as approximately 6 h after silicon repletion or light irradiation, and labeling was markedly intensified by 18 h. It is revealed that the synthesis of girdle bands begins before valve synthesis in this species, with active valve synthesis occurring during the G2 / M phase. Expression profiling revealed that selective member(s) of the F. cylindrus SIT genes (FcSIT) respond to silicate and light, with a different set of genes being responsive to each factor. The Si / light double depletion experiments demonstrated that expression of one FcSIT gene is possibly correlated to transition to G2 / M phase of the cell cycle, when the valve is actively formed. | ||
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