국가과학기술지식정보서비스

국가연구개발성과정보

성과
논문 (학술지)

Rational design of bienzyme nanoparticles-based total cholesterol electrochemical sensors and the construction of cholesterol oxidase expression system

저자, 논문 구분, 저자, 학술지명, ISSN, SCI(SCIE포함) 구분, 학술지 출판 일자, 볼륨, 페이지, 학술지 임팩트 팩터, 학술대회명, 학술대회 개최국, 키워드 항목으로 구성된 논문 상세조회를 제공하고 데이터가 없는 항목에 대해서는 출력
등록번호 - SCI 구분?
 ※구분 : SCI(SCIE포함), 비SCI
SCI
저자명 (주·공동저자) -;
논문구분 국외전문학술지 학술지명 SENSORS AND ACTUATORS B-CHEMICAL
ISSN 0925-4005 학술지 출판일자 2021-12-15
학술지 볼륨번호 349() 논문페이지 1307421 ~ 13074212
학술지 임팩트팩터 7.46 기여율 0 %
초록
In this study, we demonstrate the first report of bienzyme nanoparticles (BENP)?based cholesterol electrochemical sensor. The developed BENP consists of cholesterol oxidase (ChOx) and horseradish peroxidase (HRP), and these enzymes were crosslinked by glutaraldehyde treatment. An alternative expression system of ChOx is also firstly introduced through this work by constructing its production system in Pichia pastoris exploiting ChOx expressing gene taken from Streptomyces sp. The obtained ChOx is then applied to synthesize BENP with HRP at different ratios to attain the best condition that can yield in enhanced enzymatic activity. To utilize the material for electrochemical sensors, the BENP is furtherly modified with cysteamine to graft thiol (-SH) group that facilitates BENP immobilization on gold electrode through Au?S bond. The kinetic study of the BENP was conducted using cholesterol and H2O2, which are substrates of the BENP constituents, ChOx and HRP, respectively. Moreover, its characterizations, catalytic activity, specificity, and selectivity toward cholesterol also has been investigated. The developed BENP-based cholesterol electrochemical sensor exhibited a long dynamic range (0?675 mg/dL) with a low detection limit (0.18 mg/dL), high selectivity, and long-term stability (> 98% of its initial activity over 25 days).
☞ 성과발생연도 2020년 이후 논문은 조사분석 미확정 정보이며, 조사분석 확정시 기여율 등 일부 정보가 변경될 수 있습니다.

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